Neurotransmitter Transporter Uptake Assay Kit. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $3,500. 0´ 104 8. 4 software package (Molecular. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. Turn on the power to the FilterMax F5. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). 4 Pages. Luminescence intensity was measured with a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. 40266 Email: [email protected] or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. 3 Pages. Manufacturer: Molecular Devices Model: FilterMax F5 Condition: Used. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated. The mixture was then boiled for 10 min. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. F5 Multi-Mode Microplate Reader. The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. The absorbance values of the results were expressed as optical density (OD) as the mean ± standard deviation. Multi-Mode Readers. Add to my manuals. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. 50/50. , Sunnyvale, CA, USA) and normalized to the absorbance in the. , Sunnyvale, CA, USA) and normalized to the. The experiment was carried out with four biological replicates and two technical replicates. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. A) CHO colonies, serum-free suspension-adapted, in CloneMedia-CHO. 5 mP. Devices FilterMax Multi-Mode Microplate Readers. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . GPCRs. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 5 million in 2019 and is projected to reach US$ 704. 00. 7. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. 4 Pages. Discover Precision and Reliability with Our Featured General Laboratory Instruments - Thermo Forma 3110 Water Jacketed CO2 Incubator, Thermo ULT185-5-V -80 Freezer, Filtermax F5 Multimode Microplate Reader, Spectramax M5e, Thermo Napco CO2 Incubator, and XS 64 Balance. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. FilterMax F5 Multi-mode microplate reader. This can increase throughput and efficiency, thus saving time and space in the lab. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. 2. Coculture experiment. SpectraMax iD5. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. Select the protocol “Concentration_pico_green_v3. The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. 40266 Email: [email protected]™ F3 and F5 Multimode Microplate Readers, Molecular Devices. 4. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seEye lysates were developed using a Glucose Colorimetric Assay Kit (Sigma, Louis, MO, USA) and measured by a FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 570 nm. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Luminoskan luminometric microplate reader. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. 00 USD. A microplate consists of small wells wherein separate reactions take place. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The increase in NADH, an end product in this reaction, was determined by. Luminescence was recorded with a luminometer (Filter Max F5 Multi-Mode Microplate reader) with SoftMax Pro Easy software, and the NADH/NAD + ratio was calculated by comparing the relative light units. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. 4 Pages. Instrument Configuration: Assay Technology Adapta® & • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. ” e. The amount of colorimetric product was continuously monitored for 120 min. Supplier: Molecular Devices. MIA PaCa-2 cells were cultured in 96-well plates with 1000 cells/well. Synergy HT laboratory equipment pdf manual download. Aspirate 150 µL from wells C5-F5 and discard. MetaMorph NX. ScanLater Western Blot Detection System. Cover plate with gas-permeable membrane and put in incubator. Firefly and Renilla luciferase relative light units were measured using the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). 100 fmol 1536-well (8 µL) 0. Caspase-3/7 activity, an indicator of apoptosis, was measured by a luminescence assay using caspase-Glo. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. 2 Temperature and cooling rate control. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Laser-aided direct metal deposition of metals and alloys. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). Molecular Devices Microplate reader VersaMax $1,495. Watson. View specifications, prices, citations, reviews, and more. FilterMax F5 Multi-Mode Microplate Reader The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence,. 2. You can determine the model and type of your F5 hardware platform through the command line or the Configuration utility. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. SoftMax Pro GxP Compliance Software. They provide absorbance, fluorescence, glow luminescence, and other read modes for. Page 38 | Getting Started Overview The Cytation 5 is a hybrid multi-mode microplate reader. 00. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. After 30 min, the reaction was stopped by adding 1 N HCl and absorbance at a wavelength of 490 nm was measured using the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. The amount of each HTRA1 protein was. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. 40266 Email: drugdiscoverytech@lifetech. 3 Pages. Place plate in the GloMax® Instrument and read. We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by normalizing to total protein. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 00 SpectraMax M3, M4, M5, and M5e Multi-Mode Document type: User Manual5016208D 8 Chapter1:Introduction TheFilterMax™ F3Multi-ModeMicroplateReaderandtheFilterMax™F5Multi-Mode MicroplateReaderareidealforabroadrangeofapplications. VANTAstar™ Flexible Multi-mode Microplate Reader. SpectraMax iD5. 3 Pages. Applications: Absorbance, Cell viability, ELISA, Fluorescence,. Plate Formats:. ** Some compatible filter sets are available for. 6 Pages. 012 fmol 0. FilterMax F3 & F5 Microplate Readers. ABS Test results. In . ** Some compatible filter sets are available for purchase from Life. A blank sample was prepared by mixing. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. Caspase 3/7 activity was performed as previously described [8,9,10,20]. ScanLater Western Blot Detection System. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. performs within its specifications. 713579). Match case Limit results 1 per page. Then, 50 µL of a stop solution were applied to terminate the reaction. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Cell growth and viability were assessed using the CCK-8 proliferation assay. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. Before unpacking and setting up the FilterMaxTM F3 or FilterMaxTM F5 Microplate Reader, prepare a dry, flat work area that has sufficient space for the instrument, host computer,. Caspase-3 Activity. 8 Pages. F3/F5 Multi-Mode Microplate Readers Fluorescent signals from the same assay plate were measured on the SpectraMax iD5 reader (the SpectraMax iD3 reader is also suitable for these assays, as it has the same onboard fluorescence performance as the SpectraMax iD5 reader), SpectraMax i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. The plates were loaded into a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA), and the fluorescence intensity was measured at 37 °C with Ex/Em = 440/484 nm and 15 s shaking between reads to facilitate aggregation. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. 3 Pages. The VICTOR Nivo multimode microplate reader is a high-performance filter-based plate reader equipped with all major detection technologies – Alpha, luminescence, etc. Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. Buffer without protease was used as a blank for background subtraction. The experiment was performed in 4 technical replicates (wells in the cell culture plate). Please note it is NOT a Keyspan USA-19 HS USB adaptor that is commonly used by. Click “Eject Plate Carrier. . Caspase 3/7 activity was measured according to previously established methods [11,12,13,15,16]. The planktonic growth inhibition (%) was calculated according to the following equation:Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. For WST-1–based assay, 10 μL ready-to-use WST-1 reagent was added to each well and incubated for 3 hours at 37°C, and absorbance at 450 nm was measured. The fluorescent signals were measured at 10 minutes and one hour after the Stoplight Red substrateFilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) 12. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by extrapolation from a standard curve made in a four-parameter nonlinear regression program in Softmax Pro software (Molecular Devices). FilterMax F5 Multi-Mode Microplate Reader. 1 at 600 nm as determined by FilterMax F5 multi-mode microplate readers (Molecular Devices). Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 100 fmol 384-well (75 µL) 0. The experiment was carried out with two biological replicates and three technical replicates. Delete from my manuals. 00. Share. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Time-resolved Fluorescence. . The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. RNA extraction and reverse transcriptase–quantitative PCR. Multi-Mode Microplate Reader. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. ABS Test results. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. The other members of the E39 family (525i, 530i, and 540i) do not have limited slip. cruzi incubated for 1 h with sertraline at 2 μM. 4. Fred A. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Molecular Devices, LLC Reading Modes: Endpoint, Kinetic, Linear Scan, Area Scan; Optical System: LEDs, Deuterium Lamp; Wavelength Range: 230 to 750 nm;. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of. 2. 3. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. SpectraMax i3x Multi-Mode Detection Platform Biotek Synergy H1 Operator's Manual Appliskan User Manual FilterMax F3/F5 Multi- Mode Microplate Readers Spectramax® m2/m2 user guide User manual Software version 3. SpectraMax iD5. 5 μg/ml) in the dark for 1 h. 5. 375% (w/v) thiobarbituric acid was added. Neurotransmitter Transporter Uptake Assay Kit. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). 9 mM H 2 O 2 for. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. Dave Zeckhausen. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. #ID5-STD), equipped with the following: Enhanced TRF Module (Molecular Devices cat. When recombinant supernatants were tested for the presence of Il10, only values within the linear range of. The. The amount of the colorimetric product was monitored continuously for 120 min. Each kit was used on the same day that its reagents were reconstituted. Transfluor Technology. MD, MPH, Milton J. ** Some compatible filter sets are available for purchase from Life. Seoul, South Korea. , San Diego, CA, USA). The data were analyzed using Multi-Mode Analysis software and normalized to fluorescence in the vehicle-treated cells (% of control). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 05); b Evaluation of the ATP levels of T. 1 at 600 nm as determined using a FilterMax F5 multi-mode microplate reader (Molecular Devices). Measurement of Caspase 3/7 Activity. Yes, the M5 does have a limited slip. Molecular Devices technical support is available by phone and e-mail. d. FilterMax F3 & F5 Multi-Mode Microplate Readers from. The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. 4 Pages. FilterMax F3 & F5 Microplate Readers. 4 million by 2027; it is expected to grow at a CAGR of 3. , Sunnyvale, CA, USA). 3. With optimized reagents, option to operate the. FilterMax F3 & F5 Multi-Mode Microplate Readers. A microplate reader (also called sometimes microtiter plate reader) is a high-sensitivity instrument designed to analyze samples contained in microplate wells. F3/F5 Multi-Mode Microplate Readers. 50/50. The OD 600 was plotted against time using Graphpad Prism 8. URL of this page: HTML Link: Bookmark this page. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Recommended Optics P/N Filter information 6590-0048 FilterMax™ Fluorescence Excitation Filter 405 nm (bandwidth 30 nm) FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate Reader Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Synergy HTX laboratory equipment pdf manual download. Devices FilterMax Multi-Mode Microplate Readers. KG (CAT#: STEM-LE-1659-Y)The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAfter 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. 3 Pages. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . MetaMorph NX. The multi-mode microplate reader provides flexibility and ease of use for a variety of applications Market Highlights Microplate Readers Market is predicted to project a notable CAGR of 4. In addition, the instrument features linear and orbital shaking, plus temperature. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. time plots at 30, 60, and 90 min using MATLAB ® R2017b (9. GPCRs. Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. microplate reader. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. Related Products: FilterMax F3 &. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. #0200-7030) Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Instrument Setup: 1. , Sunnyvale, CA, USA) and. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. After the mixture was cooled, TBARS was measured at 532 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Reader, Molecular Devices). This document is. SpectraMax iD3 and iD5 Multi-Mode Microplate Readers;. 1 Software Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. ~ Bio-Tek ELx808 Microplate Reader $2,795. , Ltd. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. Read Review (1)FlexStation® 3 Multi-Mode Microplate Reader. Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3. Microplate Readers. HERM LB 500 with NaI detector Radio HPLC Detector. BioStack Neo is a dedicated stacker offering super-FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. To do so, perform one of the. Neurotransmitter Transporter Uptake Assay Kit. Optical densities (OD) from ELISA assays were measured with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). The OD 595 of the culture in the plate (light path length: ∼6. Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). Manufacturer: Molecular Devices. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. The 50% inhibitory concentration (IC 50) values were determined using the nonlinear regression curve fit analysis of Prism 9. The increase in NADH, an end product in this reaction, was determined by means of its light absorbance at 355 nm using a fluorometric microplate reader (FilterMax F5 Multi-Mode Microplate Reader; Molecular Devices,. The hemolysis was calculated with the following formula:Luminescence from the converted ATP was then measured using a FilterMax F5 Multi-mode microplate reader (Molecular Devices). ImageXpress Micro 4 High-Content Imaging System. 3 Pages. CloneMatrix. The amount of the colorimetric product was monitored continuously for 120 min. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. #6590-0118) Filter 665/10 (Molecular Devices cat. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 3 Pages. ] Example of document to evaluate if the reader . Supplier: Molecular Devices. ScanLater Western Blot Detection System. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 3 Pages. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Sign In Upload. 425 or. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. Add to cart. (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular devices, CA, USA). The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Chemiluminescence measurements were performed using a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 1 (GraphPad Software Inc. This document is provided to customers. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Download Table of Contents. CatchPoint cGMP Detection Assay Kit. 425 or. Neurotransmitter Transporter Uptake Assay Kit. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. At least three independent experiments were performed for each. Absorbance was measured at 450 nm using a FilterMAx F5 Multi-Mode Microplate Reader and EX2 filter after a 2-hour incubation at 37°C with 10 μL of CCK-8 per 100 μL of media. FilterMax F3 & F5 Microplate Readers. Description Z-height focusing is enabled for fast top- and bottom-reads for all read modes. Activation of TLR4 and TLR2 receptors. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. ScanLater Western Blot Detection System. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 3 Pages. Time-resolved Fluorescence. Search LabX. Triplicates of. ScanLater Western Blot Detection System. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The inter-assay coefficient of variations (CV) and values for all biomarkers can be found in Table 4. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell. Molecular Devices’ FilterMaxTM F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices SpectraMax 340PC384 Microplate Reader, Molecular Devices. The capability of the lixiviated products of the experimental membranes to inhibit the bacterial planktonic growth was estimated from the turbidity of the inoculated culture media at OD λ = 595 nm (filter max F5 multi-mode microplate reader). Cells were seeded in 24-well plates and cultured. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceF3/F5 Multi-Mode Microplate Readers. 2 Pages. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. 2 μM ATP standard was. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. The readings of samples incubated with different carbon sources were subtracted from the average of the control readings. The following multi-step binary elution gradient, based on an water as phase A and acetonitrile as phase B, both containing 0. If using a multi-functional reader, please be sure to check the sensitivity of the device using the MycoAlert™ Assay Control Set. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. BMG Labtech - THERMOstar. Read Review (1)The mixture were then centrifuged at 1000 g for 5 min. Category 2 and 3 microplate readers use gradient heating as well. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. The supernatant was taken and 0. Immunoblot analysis was performed with pooled serum (diluted 1:500) separated by SDS-PAGE under non-reducing conditions and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. Catalog. 4 (Molecular Devices, Sunnyvale, CA, USA) at room temperature (fluorimeter settings used were as follows: measurement type, kinetic, 60 cycle, 60-s interval; excitation wavelength, 360 nm;. 2 Pages. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Other recommended products. MetaMorph NX. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. The colorimetric signal development was stopped after 10 minutes.